|Xuemei Cao, Xiao Liu, Hongda Li, Yumeng Fan, Jiabin Duan, Yi Liu, Qun He. Transcription factor CBF-1 is critical forcircadian gene expression by modulating WHITE COLLAR complex recruitment to thefrq locus. PLOS Genetics. DOI: 10.1371/journal.pgen.1007570|
Transcription factor CBF-1 is critical forcircadian gene expression by modulating WHITE COLLAR complex recruitment to thefrq locus
Xuemei Cao, Xiao Liu, Hongda Li, Yumeng Fan, Jiabin Duan, Yi Liu, Qun He
Transcription of the Neurospora crassa circadian clock gene frequency (frq) is an essentialprocess in the negative feedback loop that controls circadian rhythms. WHITE COLLAR 1(WC-1) and WHITE COLLAR 2 (WC-2) forms the WC complex (WCC) that is the main activatorof frq transcription by binding to its promoter. Here, we show that Centromere BindingFactor 1 (CBF-1) is a critical component of the N. crassa circadian clock by regulating frqtranscription. Deletion of cbf-1 resulted in long period and low amplitude rhythms, whereasoverexpression of CBF-1 abolished the circadian rhythms. Loss of CBF-1 resulted in WC-independentFRQ expression and suppression of WCC activity. As WCC, CBF-1 also bindsto the C-box at the frq promoter. Overexpression of CBF-1 impaired WCC binding to the Cboxto suppress frq transcription. Together, our results suggest that the proper level of CBF-1 is critical for circadian clock function by suppressing WC-independent FRQ expressionand by regulating WCC binding to the frq promoter.